Isolation of proteins from in vitro dengue virus type-2 infected HEPG2 cells treated with natural purified product / Tuan Fatin Farhana Tuan Mat

Dengue has been a global burden particularly to countries in tropical and subtropical region for several decades. Dengue virus infection is one of the most important arthropod-borne disease worldwide, with an estimated 50 million cases per year and 2.5 billion people are living in area with high risk of infection. Dengue has a wide spectrum of clinical presentation. It may present asymptomatically, undifferentiated febrile illness (viral syndrome), dengue fever (DF), or in more severe cases it may feature as dengue haemorrhagic fever (DHF) including dengue shock syndrome (DSS). Patients with dengue may develop high grade fever with headache, maculopapular rash, myalgia, arthralgia, retoorbital pain and bleeding through orifices. The effort in dengue control includes vector control and the most recent effort is the search for antiviral agents. Every step of the viral life cycle is a potential target for antiviral intervention. Non-structural proteins, NS3 and NS5 have been considered as the target for antiviral drugs. The interest of using natural product as an antiviral drugs has been growing dramatically especially in low income countries. This study was based on the previous findings of the inhibitory properties of pinostrobin towards DENV2.