Comparison of different reverse transcriptase to generate full-length mRNA transcript by CapFinder method / Noor Haslina Zainor Abidin

The CapFinder method is a method to amplifiy full-length mRNA transcripts. CapFinder technology is a much simpler method and it requires a cDNA synthesis reaction and PCR reaction to amplify the desired 5’ or 3’ ends of a cDNA. The major objective of this study is compare three different reverse transcriptases from three different point-mutant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase which are SMARTScribe Reverse Transcriptase (Clontech), SuperScript III Reverse Transcriptase (Invitrogen) and RevertAid H Minus Reverse Transcriptase (Fermentas) to generate full-length mRNA transcript via capFinder method. The study will compare these three reverse transcriptases that vary with price range with regards to sensitivity and efficiency in generating full-length first-strand cDNA by agarose gel analysis of the amplified cDNA.