The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah

Kamal Basah, Norizan (2017) The effect of leptin on utero-placental expression of EPHB4, Ephrin-B1, Ephrin-B2 and blood pressure in Sprague Dawley Rats / Norizan Kamal Basah. Masters thesis, Universiti Teknologi MARA (Kampus Sg. Buloh).


Background: Ephs and ephrins might play a role in the migration of cytotrophoblast during endometrial invasion. Objective: This study attempts to establish the pattern of expression of EPHB4, Ephrin-B1 and Ephrin-B2 in the utero-placental unit during pregnancy in control and leptin-treated rats. Method: Normotensive female Sprague Dawley rats, aged 12 weeks in pro-estrous were individually housed overnight with a male. Upon confirmation of mating the rats were randomized into two groups; control group and leptin-treated group. These groups were further divided into 10 subgroups (n=6 per group). Rats in the leptin-treated groups were given daily 60 µg/kg BW/day of leptin subcutaneously from two weeks prior to mating and until the day they were euthanized. Rats were euthanized every 2 days from day 5 of pregnancy and the placentae were collected for RT-PCR and immunohistochemistry. Data were analyzed using two-way ANOVA and expressed as mean ± SEM. Result: Results showed that Ephrin-B1 and EPHB4 expressions in the placenta and uterus decreased significantly from early to mid-pregnancy and then increased towards the end of pregnancy in normal rats (p<0.001). Ephrin-B2 expression decreased over the course of pregnancy in both the placenta and uterus (p<0.001). Ephrin-B1 expression was significantly higher in the leptin-treated group at day 21 of pregnancy. It appears that Ephrins-B1, B2 and EPHB4 are expressed by both the placenta and the uterus of the rat and their expression varies along the course of pregnancy. Conclusion: In conclusion, although the expression of Ephrin-B1 is affected by leptin, it might not have a role in leptin induced hypertension during pregnancy in the rats.


Item Type: Thesis (Masters)
Email / ID Num.
Kamal Basah, Norizan
Email / ID Num.
Thesis advisor
Sergey, Gupalo
Subjects: Q Science > QL Zoology > Chordates. Vertebrates
Q Science > QP Physiology > Animal biochemistry > Organic substances
R Medicine > RC Internal Medicine > Specialties of internal medicine > Diseases of the circulatory (Cardiovascular) system
Divisions: Universiti Teknologi MARA, Selangor > Sungai Buloh Campus > Faculty of Medicine
Programme: Master of Science (Medicine)
Keywords: Leptin, Utero-Placental Expression, EPHB4, Ephrin-B1, Ephrin-B2 A, Sprague Dawley Rats
Date: July 2017
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