Reprogramming foreskin fibroblast cells from haemophilia Type-A patients into induced pluripotent stem cells / Noor Sabirah Husin

Induced Pluripotent Stem Cells (iPS cells) has an enormous potential in clinical application for example in the modelling of haemophilia. Studies on human pluripotent stem cells give hope to formulate potential cellular therapies for disease modelling. This study was designed to reprogram fibroblast cells from haemophilia type-A patients into iPS cells using four different techniques. Enzyme dissociation and explant culture technique were used to isolate primary cell fibroblast from haemophilia type-A patient. The enzymatic dissociation technique required three days to develop into fibroblast cells compared to explant culture which required 10 days in culture. Properties of the iPS cells depended on the reprogramming method. Morphological characteristics of iPS cells were observed only in Stemgent mRNA and Neon ® Reprogramming techniques. Expressions of pluripotency were verified using pluripotency markers. Downregulated and upregulated gene expression were analysed using qRT-PCR. This study provides new information on the development of haemophilia modelling, based on patientspecific iPS cells using integration-free methods.