Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]

Azmi, Azlin Suhaida and Mohd Bakari, Nur Atikah and Ali, Fathilah and Mohamad Puad, Noor Illi and Ahmad, Farah (2022) Growth kinetic of rhizopus sp. immobilised on loofah sponge for whole-cell biocatalyst in different cultivation media / Azlin Suhaida Azmi … [et al.]. Malaysian Journal of Chemical Engineering and Technology (MJCET), 5 (1): 5. pp. 38-42. ISSN (e-ISSN): 2682-8588

Abstract

Immobilisation of filamentous fungi such as Rhizopus sp. onto biomass support particles (BSP) surface was studied as whole-cell biocatalyst. The function was to aid fermentation process. By using the microbial immobilisation process, the complex procedures of isolation, purification and immobilisation of extracellular enzyme can be avoided. Loofa sponge was selected as the BSP to aid in the immobilisation of the cells. In this study, the growth of immobilised Rhizopus sp. on loofa sponge was compared in four different cultivation media and the attachment of Rhizopus sp. on loofa sponge was investigated using scanning electron microscopy (SEM). The media used in this study were hydrolysed cassava starch, cassava dextrose, potato dextrose, and soy dextrose. The process condition and other parameters which were temperature, pH, inoculum dilution, and weight of loofa sponge, fixed at 30 °C, 7, 20 mL, and 2 g, respectively. The highest and lowest of maximum growth (ymax) of the immobilised cells were determined from potato dextrose and soy powder mixed with dextrose media, respectively at 1.5281 g/g and 1.0370 g/g. Whilst, the highest and lowest observed rate constant (k) were obtained from cassava starch mixed with dextrose and soy powder mixed with dextrose, which respectively at 2.9403 day−1 and −0.8763 day−1. SEM images showed the presence of mycelia attached to the loofah sponge after immobilisation process. In conclusion, Rhizopus sp. has been successfully immobilised on loofah sponge as a whole-cell biocatalyst.

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Azmi, Azlin Suhaida
azlinsu76@iium.edu.my
Mohd Bakari, Nur Atikah
UNSPECIFIED
Ali, Fathilah
UNSPECIFIED
Mohamad Puad, Noor Illi
UNSPECIFIED
Ahmad, Farah
UNSPECIFIED
Subjects: T Technology > TP Chemical technology
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Chemical Engineering
Journal or Publication Title: Malaysian Journal of Chemical Engineering and Technology (MJCET)
UiTM Journal Collections: UiTM Journal > Malaysian Journal of Chemical Engineering and Technology (MJCET)
ISSN: (e-ISSN): 2682-8588
Volume: 5
Number: 1
Page Range: pp. 38-42
Keywords: Fermentation, Immobilisation, Loofa sponge
Date: April 2022
URI: https://ir.uitm.edu.my/id/eprint/60412
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