Transgenic studies of oil palm DNA in tomato / Nurul Afiqah Md Yusop and Yusuf Chong Yu Lok

Tomato, scientifically named (Solarium Lycopersicon L.) is one of the most important vegetable crops and a model plant to study other plants. For further study in biotechnology especially for basic and practical studies for tomato improvement, successful transformation is essential. However, the success of genetic manipulation of a plant depends on the transformation as well as regeneration frequencies of the explants. So the purpose of this study is to assess and compare different transformation protocols in tomato. Transformation of cotyledonary leaf explant was successfully performed with gene transfer into tomato by Agrobacterium tumefaciens mediated natural transformation and strain LBA4404. Frequency of transient and stable expression has been identify and showed that the transformation competence in tomato was highly influenced by several factors for example Agrobacterium suspension. There-are nine factors affecting the efficiency of tomato transformation including genotypes, explant type, agrobacterium strains, explant size, explant orientation on medium, array, co-cultivation period, pre-culture of explant and kanamycin concentration tolerance test for selection of transformed tissue. All these factors along with kanamycin concentration will determine the successful of genetic in tomato. Besides, previous study' had reveal that 200 mg/1 kanamycin is optimum for selection of transformed explant. This review also discovered that, in-vitro fruit injection (in-planta transformation) method was found to be more efficient transformed plants as compared to conventional tissue culture based transformation methods.