Simultaneous virological and bacteriological screening of microbial isolates in Perna viridis / Ernie Suhaiza Radzi ... [et al.]

Radzi, Ernie Suhaiza and Mohamed, Emida and Rambely, Azlin Sham and Haron, Norhisham and Camalxaman, Siti Nazrina (2019) Simultaneous virological and bacteriological screening of microbial isolates in Perna viridis / Ernie Suhaiza Radzi ... [et al.]. Health Scope, 1. pp. 48-51. ISSN 2735-0649

Abstract

The global consumption of shellfish has increased over the past few years concurrent with reports of viral and bacterial gastroenteritis outbreaks worldwide. This study was conducted to screen both viral and bacterial agents in Perna viridis using Transmission Electron Microscope (TEM) and molecular based detection methods. Direct genomic extraction methods were tested on mussels, which were purchased from Shah Alam wet market. Samples were subjected to Reverse Transcription-Polymerase Chain Reaction (RT-PCR) for viral screening and conventional PCR for bacterial detection. In this study, no viral agent was discovered but PCR amplification of the lamB gene yielded a single band at 309 bp, identifying E. coli as being present in Perna viridis tissues. Continuous monitoring of shellfish for pathogens will help ensure its safety for human consumption. Further studies focusing on the relationship and quantitative measurement of fecal indicator bacteria in coastal areas/aquaculture settings should be addressed.

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Radzi, Ernie Suhaiza
UNSPECIFIED
Mohamed, Emida
UNSPECIFIED
Rambely, Azlin Sham
UNSPECIFIED
Haron, Norhisham
UNSPECIFIED
Camalxaman, Siti Nazrina
sitinazrina@uitm.edu.my
Subjects: Q Science > QR Microbiology > Microbial ecology
Q Science > QR Microbiology > Virology
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Health Sciences
Journal or Publication Title: Health Scope
UiTM Journal Collections: Others > Healthscope
ISSN: 2735-0649
Volume: 1
Page Range: pp. 48-51
Keywords: E. coli, PCR, Perna viridis, RT-PCR, TEM
Date: June 2019
URI: https://ir.uitm.edu.my/id/eprint/54757
Edit Item
Edit Item

Download

[thumbnail of 54757.pdf] Text
54757.pdf

Download (189kB)

ID Number

54757

Indexing

Statistic

Statistic details