Leaves aqueous extract as a cytological stain for buccal cell screening / Mohd Nazri Abu ... [et al.]

Abu, Mohd Nazri and Zaimy, Nurizan and Sahlan, Suwadi Aryadiy and Zulkifle, Nur Atikah and Mohd Azlan, Siti Sarra Hazwani (2019) Leaves aqueous extract as a cytological stain for buccal cell screening / Mohd Nazri Abu ... [et al.]. Health Scope, 1. pp. 71-75. ISSN 2735-0649


This study describes the preparation and use of Alternanthera dentata (purple knight), Alternanthera ficoidea (grenadine), Brassica oleracea (red cabbage), Amaranthus dubius (red spinach) and Lawsonia inermis (henna) for the differential staining of buccal cells to find out its staining effect on nuclear and cytoplasm as an alternative to Papanicolaou stain in cytology laboratory. The leaves aqueous extracts were tested with or without addition of Aluminium chloride (AlCl3) mordant using regressive Papanicolaou staining procedure as a standard with minor modification. All the leaves extract was measured for pH, physical colour concentration, qualitative and quantitative staining intensity and ability. Statistical data were calculated by using Weighted-Kappa analysis showed a fair correlation between staining intensity of leaves aqueous extract dye with Papanicolaou stain while, One-way ANOVA showed no significant difference between leaves aqueous extract and Papanicolaou stain. Therefore, the leaves aqueous extracts stain were not comparable to the standard. As a conclusion, the leaves aqueous extract used in this study has the ability to stain both nucleus and cytoplasm by its own colour; however, the colour intensity is incomparable with Papanicolaou stain as the standard.


Item Type: Article
Email / ID Num.
Abu, Mohd Nazri
Zaimy, Nurizan
Sahlan, Suwadi Aryadiy
Zulkifle, Nur Atikah
Mohd Azlan, Siti Sarra Hazwani
Subjects: Q Science > QH Natural history - Biology > Cytology
S Agriculture > SB Plant culture > Field crops
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Health Sciences
Journal or Publication Title: Health Scope
UiTM Journal Collections: Others > Healthscope
ISSN: 2735-0649
Volume: 1
Page Range: pp. 71-75
Keywords: Alternanthera dentata (purple knight), Alternanthera ficoidea (grenadine), Amaranthus dubius (red spinach), Brassica oleracea (red cabbage), Lawsonia inermis (henna) Papanicolaou stain
Date: June 2019
URI: https://ir.uitm.edu.my/id/eprint/54746
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