Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri

Fish protein hydrolysate was prepared from Sillago Banded Whitings fish (Sillago argentifasciata). Hydrolysis condition such as pH and enzyme substrate ratio was used for preparing fish protein hydrolysate which were optimised by response surface methodology (RSM) using a factorial design. Model equation was proposed, with regard to the effect of pH and enzyme substrate ratio. The optimum percentage of nitrogen recovery (%NR) achieved was about 13.35% with optimum pH of 6.79 and optimum enzyme substrate ratio of 2.77% using alcalase. An enzyme substrate ratio of 0.33-% and pH of 9.00 were found to be the optimum conditions to obtain a high degree of hydrolysis close to 0.59% using alcalase. The hydrolysate prepared from Sillago banded whiting fish has potential for applications in aquaculture or animal feeds and also has a potential to be an effective nitrogenous source in microbiological growth media. Sillago banded whiting fish hydrolysate also has potential to be commercialise as a flavour enhancer.