Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak.

Abd Razak, Nurulain (2014) Characterization and purification of protease extracted from "noni" (Morinda Citrifolia L.) at two maturity stages / Nurulain Abd Razak. Degree thesis, Universiti Teknologi MARA.

Abstract

Protease was extracted from two maturity stages of noni fruits (Morinda citrifolia L.), unripe (stage 1) and ripe (stage 5) and then purified using acetone precipitation, dialysis, gel filtration (Sephadex G-25) and freeze drying. Protease obtained from each of purification step "was analysed for protein concentrations, proteolytic activity, yield and molecular weight distribution (SDS-PAGE). Freeze dried protease were analysed for pH stability, temperature stability and storage efficiency. Protein content in unripe (stage 1) noni fruit was higher than those in the ripe (stage 5) fruit. Protein concentration for protease for unripe (stage 1) noni fuit was higher for protease purified from crude extract until dialysis step. Molecular weight distribution of purified proteases for both stages was approximately at 3 to 28 kDa. The optimum activity of purified proteases was at pH 7 and 6 at 40 and 50 °C for unripe (stage 1) and ripe (stage 5) noni fruit respectively. Protease from noni fruit could be an alternative source of plant protease based "on its high proteolytic activity and stability during storage.

Metadata

Item Type: Thesis (Degree)
Creators:
Creators
Email / ID Num.
Abd Razak, Nurulain
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Ismail, Normah (Dr.)
UNSPECIFIED
Subjects: Q Science > QK Botany > Plant anatomy
Q Science > QK Botany > Plant physiology > Physical plant physiology > Growth. Development. Including pattern formation
Q Science > QK Botany > Plant physiology > Botanical chemistry. Phytochemicals
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Food Science and Technology
Keywords: Protease extracted, Noni fruits, Maturity stages
Date: 2014
URI: https://ir.uitm.edu.my/id/eprint/53058
Edit Item
Edit Item

Download

[thumbnail of 53058.pdf] Text
53058.pdf

Download (130kB)

Digital Copy

Digital (fulltext) is available at:

Physical Copy

Physical status and holdings:
Item Status:

ID Number

53058

Indexing

Statistic

Statistic details