Optimization of the hydrolysis condition for the production of ikan bolos (Sillago argentifasciata) hydrolysate by using Alcalase: effect on temperature and hydrolysis time / Noorul Fhadila Kamarudin

The optimization of the hydrolysis condition for the production of ikan bolos hydrolysate by using Alcalase were studied. Hydrolysis conditions were optimized by using a response surface methodology (RSM). The model equations were proposed with regard to the effects of temperature (T), time (t) on the percentages of nitrogen recovery (NR) and percentages of degree of hydrolysis (DH). By maintaining the pH constant at 6.5 and enzyme substrate ratio at 2%, the optimum conditions as suggested by the RSM are at 60. 26 °C which required 88.91 min of hydrolysis time in which maximum percentage of nitrogen recovery at 2.8 can be recovered with desirability of 1 and maximum percentage of degree of hydrolysis at 3.5 can be obtained at 50°C in 180 min with 0.777 desirability.