Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif.

Ahmad Latif, Nurul Nadiah (2009) Optimization of hydrolysis conditions for the production of shrimp hydrolysate by using protamex: effect of enzyme substrate (ES) ratio and time / Nurul Nadiah Ahmad Latif. Degree thesis, Universiti Teknologi MARA.

Abstract

The optimization of hydrolysis conditions which are enzyme substrate ratio ..and hydrolysis time for the production of shrimp hydrolysate by using protamex were studied. This study was done to obtain the optimum percentage for nitrogen recovery and degree of hydrolysis on the hydrolysis conditions. Methods for production of shrimp hydrolysate include homogenization, adjustment of pH and temperature, addition of enzyme and hydrolysis time which are based on response surface methodology (RSM) suggestion, termination of hydrolysis, cooled and centrifuged the hydrolysis sample, collection of supernatant and analysis for percentage of nitrogen recovery and degree of hydrolysis. Total nitrogen in supernatant was determined by using Kjeldhal method. The percentage of degree of hydrolysis was determined by using modified formol titration method. The data that were obtained from the experiment were accomplished by employing the RSM using Design Expert 4 Software. By fitting the experimental data to the equation, the percentage nitrogen recovery (%NR) optimum levels for time (240 min), ES ratio (0%), (%NR) (3.90308) with desirability (0.657) at constant pH 6 and constant temperature 60°C were determined. For percentage degree of hydrolysis (%DH), the optimum levels for time (0 min), ES ratio (4%), %DH (2.80186) with desirability (0.693) were determined.

Metadata

Item Type: Thesis (Degree)
Creators:
Creators
Email / ID Num.
Ahmad Latif, Nurul Nadiah
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Ismail, Normah (Dr.)
UNSPECIFIED
Subjects: T Technology > TP Chemical technology > Chemical engineering
T Technology > TP Chemical technology > Chemical engineering > Hydrolysis
T Technology > TP Chemical technology > Biotechnology
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Food Science and Technology
Keywords: Hydrolysis, Protamex, Enzyme substrate
Date: 2009
URI: https://ir.uitm.edu.my/id/eprint/53000
Edit Item
Edit Item

Download

[thumbnail of 53000.pdf] Text
53000.pdf

Download (136kB)

Fulltext

Fulltext is available at:
  • Koleksi Akses Terhad | PTAR Utama | Shah Alam

ID Number

53000

Indexing

|

Statistic

Statistic details