Mass spectrometry-based proteomic investigation of heterogeneous biofilms: a review / Mohamad Fakhri Yaacob … [et al.]

Raja Yahya, Mohd Fakharul Zaman and Johari, Nur Anisah and Kamaruzzaman, Alya Nur Athirah and Yaacob, Mohamad Fakhri (2021) Mass spectrometry-based proteomic investigation of heterogeneous biofilms: a review / Mohamad Fakhri Yaacob … [et al.]. Scientific Research Journal, 18 (2): 21. pp. 67-87. ISSN 2289-649X

Official URL:


Biofilm represents a major public health concern. It is a highly structured and heterogeneous microbial population that is well protected by a hydrated extracellular matrix. In most cases, the difficulties in combating a wide spectrum of biofilm-associated diseases are due to the presence of dormant cells and differential molecular expression. Proteomics is the large-scale and systematic study of cellular proteome expression at any given time by mass spectrometry. It allows high-sensitivity and high-specificity identification of differentially expressed proteins in the biofilms. Over the past few decades, multiple lines of proteomic works have successfully elucidated various aspects of the biofilm including developmental stages, antimicrobial resistance, and survival mechanisms. However, the heterogeneity of biofilms may contribute to inconsistent proteome expression throughout a proteomic experiment. This is due to the fact that the mature biofilm is often associated with the mixture between monolayer and multilayer biofilms, thick microbial population, and chemical gradient of nutrients. This review highlights the biofilm heterogeneities, the principle of mass spectrometry in proteomics, and the possible strategies for quantitative proteomic analysis of heterogeneous biofilms. It is suggested that isolation of monolayer biofilm, laser capture microdissection, flow cytometry, and subtractive proteome profiling may be considered for an accurate and reliable quantitative proteomics experiment.


Item Type: Article
Email / ID Num.
Raja Yahya, Mohd Fakharul Zaman
Johari, Nur Anisah
Kamaruzzaman, Alya Nur Athirah
Yaacob, Mohamad Fakhri
Subjects: Q Science > QP Physiology
Divisions: Universiti Teknologi MARA, Shah Alam > Research Management Centre (RMC)
Journal or Publication Title: Scientific Research Journal
UiTM Journal Collections: UiTM Journal > Scientific Research Journal (SRJ)
ISSN: 2289-649X
Volume: 18
Number: 2
Page Range: pp. 67-87
Keywords: biofilm, proteomics, mass spectrometry
Date: August 2021
Edit Item
Edit Item


[thumbnail of 51122.pdf] Text

Download (778kB)

ID Number




Statistic details