Comparative study of commercial hospital disinfectant (dettol) & ethanolic extraction of lowsonia inermis as surface disinfectant / Amirah Nurulhuda Ahmad Jais

Ahmad Jais, Amirah Nurulhuda (2019) Comparative study of commercial hospital disinfectant (dettol) & ethanolic extraction of lowsonia inermis as surface disinfectant / Amirah Nurulhuda Ahmad Jais. Degree thesis, Universiti Teknologi MARA ( Kampus Puncak Alam ).

Abstract

Surface disinfectants are an important to control the widespread of pathogens within clinical laboratory facilities, these products must have an appropriate spectrum of antimicrobial activity. However, many other factors must also be considered including the effect on human health and environmental safety. This experimental study was conducted to compare the effectiveness of the antimicrobial action between L. inermis ethanolic extraction with Dettol and also to determine the efficacy of L. inermis ethanolic extraction against common nosocomial pathogen, which is Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli. In brief, 25 g of L. inermis powder was soaked into 250 ml of 95% ethanol for three days with frequent agitation. Then, the mix was undergoing two times of filtration. Firstly, the extract was filtered by using sterile filter cloth, then followed by filtering the extract with No 1 Whatman filter. Next, to get crude, the filtrates proceed with the evaporation step by using a rotary evaporator. Ethanolic extract of L. inermis and Dettol in different concentrations were prepared and agar diffusion method test was performed. According to the findings of the antibacterial assay, ethanolic extract of the L. inermis and Dettol showed antimicrobial activity against S.aureus starting from 1.56 to 100% concentration with mean zone of inhibition of 14 to 26.5 and 14 to 28 mm respectively. As for S. epidermidis, L. inermis extract showed better inhibitory activity from 0.78 to 100% with mean of inhibition between 11 to 33 mm while 1.56% (15 to 35 mm) for Dettol. For Gram negative organisms, L. inermis extract show inhibitory activity ranging from 25 to 100% with mean zone of inhibition of 13.5 to 19.5 mm while Dettol need 100% concentration to be effective against P. aeruginosa with 11 mm mean of inhibition, whereas for E .coli, Dettol react effectively starting from 1.56% concentration with 10.5 to 24.5 mm in diameter compared to L. inermis extract that effective at 50% concentration with mean zone of inhibition of 14.5 to 18 mm. By using Independent sample t-test, with p-value more than 0.05, the results show no significant difference of L. inermis and Dettol in inhibiting the growth of tested organisms. Then, the One-Way ANNOVA test shows there is a significant difference somehow among the mean zone of inhibition of L. inermis on the four tested organisms, (p = 0.03). Based on the present finding, L. inermis extract might be a good candidate in the search for a natural disinfectant as a substitute to commercial disinfectant.

Metadata

Item Type: Thesis (Degree)
Creators:
Creators
Email / ID Num.
Ahmad Jais, Amirah Nurulhuda
2015430488
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Rambely, Azlin Sham
UNSPECIFIED
Subjects: R Medicine > RA Public aspects of medicine > Public health. Hygiene. Preventive Medicine
R Medicine > RA Public aspects of medicine > Medical centers. Hospitals. Dispensaries. Clinics
T Technology > TP Chemical technology > Chemicals
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Health Sciences
Programme: Bachelor of Medical Laboratory Technology
Date: 2019
URI: https://ir.uitm.edu.my/id/eprint/50738
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