Extracellular metabolites profile of different stages colorectal cancer cell lines / Hazwani Mohd Yusof … [et al.]

Mohd Yusof, Hazwani and Ab-Rahim, Sharaniza and Wan Ngah, Wan Zurinah and Nathan, Sheila and A Jamal, A Rahman and Mazlan, Musalmah (2021) Extracellular metabolites profile of different stages colorectal cancer cell lines / Hazwani Mohd Yusof … [et al.]. Science Letters, 15 (2). pp. 27-41. ISSN 1675-7785


Metabolic footprinting involves the determination of metabolites excreted or secreted by the cells. This study aimed to identify the differential extracellular metabolites in colorectal cancer (CRC) cells for the determination of molecular changes that occur as CRC progresses. CRC cells at different stages ie; SW 1116 (stage A), HT 29 and SW 480 (stage B), HCT 15 and DLD-1 (stage C), and HCT 116 (stage D) were grown in culture. The media in which the cells were grown are subjected to metabolomics profiling using Liquid Chromatography Mass SpectrometryQuadrupole Time of Flight (LC/MS Q-TOF). Statistical and metabolic pathway analysis was performed using Metaboanalyst software and identification of metabolites was determined by the METLIN database. A total of 27 differential extracellular metabolites were identified in CRC cells of different stages compared to stage A cells. Data from the Partial least squares-discriminant analysis (PLS-DA) score plot shows a clear separation between CRC cells of different stages with a few overlaps between stage B and C. Further analysis using variable importance in projection (VIP) revealed 14 differential extracellular metabolites that were most significant in differentiating CRC cells of the advanced stages from stage A which are 5-hydroxy-L-tryptophan, indoleacetaldehyde, 4,5-dimethylthiazole, 8-oxodiacetoxyscirpenol, bisnorbiotin, 5-amino-6- (5'phosphoribosylamino) uracil, glyceryl 5-hydroxydecanoate, sphinganine, 8,8-diethoxy-2,6- dimethyl-2-octanol, l-cystine, thiamine acetic acid, phytosphingosine, PE (20:4(5Z,8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), N-(2R-hydroxypentacosano-yl)-2Samino-1,3S,4R-octadecanetriol. The different expressions of metabolites may indicate altered metabolic pathways in the more advanced CRC cells compared to stage A. This study highlights the importance of conducting both metabolomics profiling of extracellular and intracellular to generate a more complete understanding on the molecular changes that occur as CRC progresses.


Item Type: Article
Email / ID Num.
Mohd Yusof, Hazwani
Ab-Rahim, Sharaniza
Wan Ngah, Wan Zurinah
Nathan, Sheila
A Jamal, A Rahman
Mazlan, Musalmah
Subjects: Q Science > QP Physiology > Nutrition
R Medicine > RC Internal Medicine > Neoplasms. Tumors. Oncology
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Applied Sciences
Journal or Publication Title: Science Letters
UiTM Journal Collections: UiTM Journal > Science Letters (ScL)
ISSN: 1675-7785
Volume: 15
Number: 2
Page Range: pp. 27-41
Keywords: Metabolomics, extracellular, metabolites, colorectal cancer, stages
Date: June 2021
URI: https://ir.uitm.edu.my/id/eprint/48229
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