Isolation of antioxidative constituents of gynura procumbens stem (Sambung nyawa) and its antibacterial potency against plant patogen / Nur Izni Ainina Alias

The study was carried out on the stem of Gynura procumbens, belongs to
the Astereceae family to isolate the antioxidative compounds and its
antibacterial potency against plant pathogen. In isolating the antioxidant
properties, several methods were conducted. The stem of G.procumbens
was extracted using three different polarities of solvents such as petroleum
ether, ethyl acetate and methanol. The methodology used were the
phytochemical analysis from the methanol extract, phytochemical screening
on TLC using certain spraying reagent, FTIR and 1H NMR analysis, as well
as disc diffusion method were conducted to assume the antioxidative
compound and antibacterial potency of G.procumbens. The phytochemical
analysis give the results that from methanol extract of G.procumbens stems
it shows the presence of tannins, saponins, steroids and triterpenoids,
cardiac glycosides and flavonoids. From phytochemical screening on TLC,
it shows that G.procumbens has the antioxidative terpenoids and alkaloids
from PE and EA extract respectively. The isolated compound that was
labelled as C7 from PE extract was alkaloid at Rf = 0.9250 and compound
C9 from EA extract was terpenoid at Rf = 0.2250. The antioxidative
compound C7 and C9 from petroleum ether and ethyl acetate extract might
be aromatic alkaloid glycosides and terpenoid glycosides respectively
proven by FTIR and 1HNMR spectrum interpretation supported by the
previous study. The most effective extract to inhibit the growth of
E.crysanthemi sp. was petroleum ether extract because it has the biggest
inhibition zone which was 8.5 mm at concentration 400 mg/ml.