Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan

Atan, Fariswan (2019) Study on the mobile phase composition and flow rate parameter for tributyrin using high performance liquid chromatography-uv detector (HPLC-UV) / Fariswan Atan. [Student Project] (Unpublished)

Abstract

The chromatographic separation for standard tributyrin was done by using high performance liquid chromatography (HPLC) equipped with ultraviolet (UV) detector. 20 µL of tributyrin standard with concentration of 0.018 rnM was injected into HPLC attached to the column Carbonl8 (Cl8) at 215 nm wavelength. Two optimization of HPLC parameters have been done in order to study the best condition for tributyrin elution. Two parameters were studied for the elution of tributyrin which are mobile phase composition and flowrate. It was found that the optimum mobile phase composition for tributyin was 50:50 acetonitrile:acetone and the optimum flowrate for tributyrin was at 0.55 mL/min. The calibration curve of standard tributyrin's range was found proportionally linear to tributyrin concentration from 0.009-0.075 mM and the value of R2 was 0.9502. Limit of detection (LOD) and limit of quantitation (LOQ) also have been conducted in this study. The value of LOD and LOQ obtained from the calculation were 0.0032 rnM and 0.0096 mM respectively.

Metadata

Item Type: Student Project
Creators:
Creators
Email / ID Num.
Atan, Fariswan
UNSPECIFIED
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Ahmad, Nor Monica
UNSPECIFIED
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QD Chemistry > Physical and theoretical chemistry
T Technology > TP Chemical technology
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Chemistry
Keywords: Study, mobile phase composition, flow rate parameter, tributyrin, high performance liquid chromatography-uv detector (HPLC-UV)
Date: 2019
URI: https://ir.uitm.edu.my/id/eprint/42449
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