Ipomea aquatica crude extract inhibits lipoxygenase, hyaluronidase and xanthine oxidase activities / Muhammad Helmi Nadri... [et al.]

Nadri, Muhammad Helmi and Cheng, Kian-Kai and Ong, Pei Ying and L., HongYeng and Othman, Nor Zalina and A. Mohd Yunos, A. Rafidah and Musa, Nur Fashya and Abdul Latiff, Norliza and Embi, Khairunnisa (2020) Ipomea aquatica crude extract inhibits lipoxygenase, hyaluronidase and xanthine oxidase activities / Muhammad Helmi Nadri... [et al.]. Journal of Academia, 8. pp. 9-14. ISSN 2289-6368

Abstract

Ipomea aquatica, locally known as water spinach, is one of the most common vegetable consumed by Malaysian. Based on previous studies, crude extract and phenolic compounds of I. aquatica exhibited several biological activities including antioxidant, anti-microbial and anti-proliferative. The presence
of phenolic compounds in I. aquatica may contributed to their ability to inhibit enzymes, chelate metals and scavenge free radicals. Currently, no study reported on anti-inflammatory activity of I. aquatica with respect to lipoxygenase, hyaluronidase and xanthine oxidase enzymes. The present
study aims to enhance current knowledge on biological properties of I. aquatica crude extract particularly on anti-inflammatory activity. Three enzymes that involve in inflammatory pathway were selected in this study including lipoxygenase, hyaluronidase and xanthine oxidase. I. aquatica was extracted in methanol and tested for lipoxygenase, hyaluronidase and xanthine oxidase at different concentrations using direct enzyme inhibition assay. Lipoxygenase, hyaluronidase and xanthine
oxidase inhibitory activities of the methanol crude extract increased with increasing concentration. Highest inhibition activity against lipoxygenase, hyaluronidase and xanthine oxidase were observed at a concentration of 1000 μg/ml with inhibition of 87.18%, 95.36% and 78.38%, respectively. Our finding in this study indicates potential anti-inflammatory activity of I. aquatica crude extract through inhibition of lipoxygenase, hyaluronidase and xanthine oxidase.

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Nadri, Muhammad Helmi
UNSPECIFIED
Cheng, Kian-Kai
UNSPECIFIED
Ong, Pei Ying
UNSPECIFIED
L., HongYeng
UNSPECIFIED
Othman, Nor Zalina
UNSPECIFIED
A. Mohd Yunos, A. Rafidah
UNSPECIFIED
Musa, Nur Fashya
UNSPECIFIED
Abdul Latiff, Norliza
UNSPECIFIED
Embi, Khairunnisa
UNSPECIFIED
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QD Chemistry > Organic chemistry
Q Science > QD Chemistry > Extraction (Chemistry)
Divisions: Universiti Teknologi MARA, Negeri Sembilan
Journal or Publication Title: Journal of Academia
UiTM Journal Collections: UiTM Journal > Journal of Academia (JoA)
ISSN: 2289-6368
Volume: 8
Page Range: pp. 9-14
Keywords: Ipomea aquatica crude, extract inhibits lipoxygenase, hyaluronidase, xanthine oxidase activities
Date: 2020
URI: https://ir.uitm.edu.my/id/eprint/41415
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