Study to localize the neuro-modulatory site of zerumbone in rats central nervous system (CNS) / Norsyazwani Samshudin

The aim of this study is to localize the functional site(s) of Zerumbone in rat CNS. Monitoring the nuclear expression of c-Fos, is an established reliable anatomical technique for the functioning mapping of the neuronal activity. In this study, we used c-Fos immunohistochemistry to identify the neuro-anatomical site of the Zerumbone rat brain and spinal cord. Control group of male Sprague-Dawly rats (six) were received intraperitoneal injection (i.p.) of normal saline and experimental rats (six) received i.p injection of Zerumbone (lOOmg/kg). The expression of c-fos protein immunoreactivity was detected in freeze frozen serial sections (40 um) in rat brain and spinal cord. The c-Fos immunostaining was identified as black spot. Statistical analysis was performed using student t-test. P