Population dynamics and biodiversity during spontaneous fermentation of Garcinia mangostana pericarps / Mohamad Sufian So’aib and Nik Abdul Hafiidz Nik Abdul Malek

Nik Abdul Malek, Nik Abdul Hafiidz and So’aib, Mohamad Sufian (2018) Population dynamics and biodiversity during spontaneous fermentation of Garcinia mangostana pericarps / Mohamad Sufian So’aib and Nik Abdul Hafiidz Nik Abdul Malek. Malaysian Journal of Chemical Engineering and Technology (MJCET), 1. pp. 14-21. ISSN 2682-8588

Abstract

This study investigates the population dynamics and microbial diversity during spontaneous fermentation of Garcinia mangostana pericarp (GMP). The spontaneous fermentation was carried out anaerobically for 100 days. Population dynamics was determined by enumerating pure isolates on different selective media. The microorganism’s identities were determined from the amplified 16S rDNA and 5.8S-ITS rDNA genes of bacteria and yeasts respectively. LAB and yeast were prevalent during fermentation at 103−104 cfu/ml and 103−107 cfu/ml, respectively. Around eight bacterial genera such as Klebsiella, Enterobactor, Mangrovibactor, Escherichia, Cronobactor, Komagataeibacter, Lactobacillus and Bacillus whereas five yeast genera Meyerozyma, Saccharomycete, Hanseniaaspora, Mycobacterium and Pichia were detected. Lactobacillus brevis and Lactobacillus plantarum were amongst LAB species identified. Lactic acid was suspected to promote LAB growth and total inhibition of pathogens after 21 days. The results highlighted potential starter culture consortium for future product development and conferred the microbiological safety of spontaneously fermented GMP product.

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Nik Abdul Malek, Nik Abdul Hafiidz
UNSPECIFIED
So’aib, Mohamad Sufian
UNSPECIFIED
Subjects: T Technology > TP Chemical technology > Chemical engineering
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Chemical Engineering
Journal or Publication Title: Malaysian Journal of Chemical Engineering and Technology (MJCET)
UiTM Journal Collections: UiTM Journal > Malaysian Journal of Chemical Engineering and Technology (MJCET)
ISSN: 2682-8588
Volume: 1
Page Range: pp. 14-21
Keywords: Garcinia mangostana pericarp, GMP, Spontaneous fermentation, PCR Lactic acid bacteria (LAB)
Date: 2018
URI: https://ir.uitm.edu.my/id/eprint/37481
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