Determination of plasmid stability, pRSOD extraction and SOD production by recombinant Escherichia coli / Wan Siti Atikah Wan Omar … [et al.]

Wan Omar, Wan Siti Atikah and Mohamed, Rosfarizan and Abdul Rahim, Raha and Ariff, Arbakariya (2008) Determination of plasmid stability, pRSOD extraction and SOD production by recombinant Escherichia coli / Wan Siti Atikah Wan Omar … [et al.]. Jurnal Gading UiTM Pahang, 12 (1). pp. 71-82. ISSN 0128-5599

Abstract

A preliminary study of Escherichia coli harbouring super oxidedismutase (ESOD) was carried out to ensure the capability of the recombinant to achieve protein production in large scale cultivation process. The objectives of this study are first, to determine the stability of the plasmid in the recombinant E. coli, and later, the pRSOD and SOD production After the confirmation of the availability of the plasmid, the determination of medium composition was performed to choose the best medium for highest dry cell weight (dcw). The findings indicate that the ESOD successfully carried the plasmid (pRSOD) compared with other control references, E. coli BL21 (DE3) pLysS and E. coli BL21 (DE3) pLysS pRSET A. The ESOD also achieved 100% stability over 48 generations. The SOD was also found capable to produce a reasonable amount of SOD. Thus, the study has established that the ESOD is clearly comparable to other recombinant E. coli and suitable to pursue large scale process for high cell density cultivation.

Metadata

Item Type: Article
Creators:
Creators
Email / ID Num.
Wan Omar, Wan Siti Atikah
atikah_bio@pahang.uitm.edu.my
Mohamed, Rosfarizan
UNSPECIFIED
Abdul Rahim, Raha
UNSPECIFIED
Ariff, Arbakariya
UNSPECIFIED
Subjects: Q Science > QH Natural history - Biology > Microscopy > Microorganisms
Q Science > QR Microbiology > Cultures and culture media. Culture technique.
Q Science > QR Microbiology > Bacteria
Divisions: Universiti Teknologi MARA, Pahang > Jengka Campus
Journal or Publication Title: Jurnal Gading UiTM Pahang
UiTM Journal Collections: Others > GADING
ISSN: 0128-5599
Volume: 12
Number: 1
Page Range: pp. 71-82
Keywords: Plasmid stability, Recombinant E. coli, Superoxide dismutase, SOD
Date: October 2008
URI: https://ir.uitm.edu.my/id/eprint/36331
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