Transmission of coconut cadang-cadang viroid oil palm variants to oil palm tissue culture plantlets / Hendry Joseph… [et al.]

Joseph, Hendry and Vandamalai, Gandesan and Abdullah, Siti Nor Akmar and Lau, Wei Hong and Randles, John W. and Abu Seman, Idris (2017) Transmission of coconut cadang-cadang viroid oil palm variants to oil palm tissue culture plantlets / Hendry Joseph… [et al.]. Borneo Akademika, 2 (1). pp. 28-34. ISSN 2462-1641


This study was conducted to transmit Coconut cadang-cadang viroid (CCCVd) variants to oil palm tissue culture plantlets. The source of inoculum was extracted by using the polyethylene glycol (PEG) extraction method from the leaflets of an oil palm with the orange spotting (OS) symptom and which has tested positive as a CCCVd oil palm variant. The concentration of leaf extract was adjusted to 20μg/ml and inoculated into 1 to 3-month-old plantlets with a high pressure injector. Six months after inoculation, the orange spotting symptom was observed on the leaflet of the plantlet. When subjected to dot blot assay, the sample hybridised with the CCCVd246 full-length complementary probe, indicating the presence of CCCVd-like RNAs in the inoculated plantlet. This suggests that the CCCVd oil palm variants have been successfully transmitted into the host. This study has demonstrated the effectiveness of using a high pressure injector in transmitting CCCVd oil palm variants and showed the potential of using oil palm tissue culture plantlet in pathogenicity studies due to benefits in terms of economies of time, space and labour.


Item Type: Article
Email / ID Num.
Joseph, Hendry
Vandamalai, Gandesan
Abdullah, Siti Nor Akmar
Lau, Wei Hong
Randles, John W.
Abu Seman, Idris
Subjects: S Agriculture > S Agriculture (General) > Soils. Soil science. Including soil surveys, soil chemistry, soil structure, soil-plant relationships
S Agriculture > S Agriculture (General) > Soil conservation and protection
S Agriculture > S Agriculture (General) > Fertilizers and improvement of the soil
Divisions: Universiti Teknologi MARA, Sabah > Kota Kinabalu Campus
Journal or Publication Title: Borneo Akademika
UiTM Journal Collections: UiTM Journal > Borneo Akademika (BA)
ISSN: 2462-1641
Volume: 2
Number: 1
Page Range: pp. 28-34
Keywords: CCCVd oil palm variants, Oil palm tissue culture plantlet, High pressure injector
Date: January 2017
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