Multiple regeneration of clinacanthusnutans nodal explants by using 6-Benzylaminopurine (BAP) Hormone / Siti Zulaiha Ghazali and Saiyidah Nafisah Hashim

Siti Zulaiha, Ghazali and Saiyidah Nafisah, Hashim (2015) Multiple regeneration of clinacanthusnutans nodal explants by using 6-Benzylaminopurine (BAP) Hormone / Siti Zulaiha Ghazali and Saiyidah Nafisah Hashim. In: Proceedings of Postgraduate Conference on Global Green Issues (Go Green), 7-8 October 2015, Dewan Kuliah Al-Khawarizmi Universiti Teknologi MARA Cawangan Perak.

Abstract

Clinacanthusnutans is a medicinal plants currently found to be able to treat cancer. This plant can be found
mostly in China, Thailand and Malaysia. Since a large portion of pharmaceutical drugs are derived, the demand
for these raw materials is steadily rising. In order to fulfill the demand on the numbers of C. nutans plant in the
drug production, multiple regenerations by plant tissue culture technique is applied and production of
undifferentiated callus were done. Culture environmental condition was controlled and sterile nodal explants
were inoculated in a MurashigeSkoog (MS) media supplemented with plant hormone cytokinin, BAP (6 –
Benzylaminopurine) at different concentrations (0 mg/L, 1.0 mg/L, 1.5 mg/L, and 2.0 mg/L). Plants were subcultured at every 4 weeks and sterile grown plants were used asexplants to grow highly proliferated callus at different media concentration. Callus inductions were also tested at two different time period. The observation of the regeneration was observed and analyzed after 8 weeks. Results showed MS media with 2.0 mg/L BAP gave the highest regeneration, mean number leaves and height of explants formation with 11.20 ± 0.374 and 5.260 ± 0.404 respectively. Besides that, at 2.0 mg/L concentration, the explants show the formation of roots after 7 weeks. The formulated media in regenerating this valuable herbal plant with anticancer beneficiary compound will give a significant contribution to the society.

Metadata

Item Type: Conference or Workshop Item (Paper)
Creators:
Creators
Email / ID Num.
Siti Zulaiha, Ghazali
ctzulaiha_91@yahoo.com
Saiyidah Nafisah, Hashim
saiyidah394@perak.uitm.edu.my
Subjects: R Medicine > RC Internal Medicine > Cancer
R Medicine > RC Internal Medicine > Cancer > Research. Experimentation
Divisions: Universiti Teknologi MARA, Perak > Seri Iskandar Campus
Event Title: Proceedings of Postgraduate Conference on Global Green Issues (Go Green)
Event Dates: 7-8 October 2015
Page Range: pp. 451-454
Keywords: Clinacanthusnutans, anticancer, 6– Benzylaminopurine, regeneration, nodal
Date: 2015
URI: https://ir.uitm.edu.my/id/eprint/33204
Edit Item
Edit Item

Download

[thumbnail of 33204.pdf] Text
33204.pdf

Download (215kB)

ID Number

33204

Indexing

|

Statistic

Statistic details