Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]

Nawi, Liliwirianis and Md Isa, Nurun Nadhirah and Musa, Nor Lailatul Wahidah and Mohd Jan, Sarah Laila and Shaikh Nasir, Nur Thabitah (2019) Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]. Gading Journal for Science and Technology, 2 (1). pp. 17-22. ISSN 2637-0018


Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family.
The objectives of this study were to determine antimicrobial activity and Minimal Inhibition
Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Methanol and hexane were the two extraction solvents that had been used. Four
concentrations of extracts; 50 mg/mL, 100 mg/mL, 200 mg/mL, and 400 mg/mL were prepared
for antimicrobial activity. Meanwhile for MIC determination, 6.25 mg/mL, 12.5 mg/mL, 25
mg/mL and 50 mg/mL concentrations were prepared. By using disc diffusion method, the
methanol extract of G. procumbens leaves showed antimicrobial activities against Staphylococcus
aureus, Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa compared to
hexane extract. Highest antimicrobial activities were recorded against S. aureus at 400 mg/mL
concentrations with 10.5 mm of inhibition zone. Broth dilution assay resulted MIC for methanol
crude extract against S. aureus, B. subtilis, K. pneumoniae, and P. aeruginosa were at 12.5 mg/mL,
25 mg/mL, and 50 mg/mL, respectively


Item Type: Article
Email / ID Num.
Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
Subjects: S Agriculture > SB Plant culture > Medicinal plants (Culture only)
T Technology > TP Chemical technology > Biotechnology
Divisions: Universiti Teknologi MARA, Pahang > Jengka Campus > UiTM Press Pahang
Journal or Publication Title: Gading Journal for Science and Technology
UiTM Journal Collections: UiTM Journal > Gading Journal of Science and Technology (GADINGS&T)
ISSN: 2637-0018
Volume: 2
Number: 1
Page Range: pp. 17-22
Keywords: Antimicrobial, G. procumbens, Minimal Inhibition Concentration (MIC), Sambung Nyawa,
Date: 31 March 2019
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