Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.]

Mohd Zain, Zaini and Johari, Muhammad Fikri and Mohd Husin, Nurul Shahirah and Rozman, Nurul Syamimi and Ab Rashid, Athirah and Bahanuddin, Latifah and Mohamad Zain, Muhammad Ilyas Danial (2017) Staphylococcus aureus nasal carriage in medical students of Universiti Teknologi MARA / Zaini Mohd Zain … [et al.]. Journal of Clinical and Health Science, 2 (1). pp. 1-6. ISSN 0127 – 984X


To determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) nasal carriage and detection of S. aureus leukotoxins among medical students of Universiti Teknologi MARA. Methods: Both sides of the anterior nares of 136 volunteers, comprising 68 preclinical and 68 clinical medical students, were swabbed and immediately cultured onto mannitol salt agar for growth of S. aureus. Standard microbiological techniques were conducted to identify and confirm the S. aureus colonies and susceptibility test against oxacillin were conducted by using Kirby-Bauer method to determine their resistance to methicillin. Polymerase chain reaction was performed for detection of leukotoxins, i.e., Panton-Valentine Leukocidin (PVL) and -haemolysin genes. Results: Nineteen students (14%) consisting of 10 preclinical (14.7%) and 9 clinical (13.2%) were nasal carriers of S. aureus. However, none of the S. aureus isolates were MRSA. No PVL gene was detected but eight of them were positive for -haemolysin gene. Conclusion: There were no MRSA nasal carriers among the medical students, but a low prevalence of S. aureus nasal carriers was detected. These carriers do not pose as high risk because none of the strains of S. aureus possess both the -haemolysin toxin and the PVL toxin that are associated with tissue necrosis.


Item Type: Article
CreatorsEmail / ID. Num
Johari, Muhammad FikriUNSPECIFIED
Mohd Husin, Nurul ShahirahUNSPECIFIED
Rozman, Nurul SyamimiUNSPECIFIED
Ab Rashid, AthirahUNSPECIFIED
Bahanuddin, LatifahUNSPECIFIED
Mohamad Zain, Muhammad Ilyas DanialUNSPECIFIED
Subjects: Q Science > QR Microbiology
R Medicine > RC Internal Medicine
R Medicine > RC Internal Medicine > Infectious and parasitic diseases
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Medicine
Journal or Publication Title: Journal of Clinical and Health Science
Journal: UiTM Journal > Journal of Clinical and Health Sciences (JCHS)
ISSN: 0127 – 984X
Volume: 2
Number: 1
Page Range: pp. 1-6
Official URL:
Item ID: 30119
Uncontrolled Keywords: Staphylococcus aureus, MRSA, nasal carriage, medical students, PVL, -haemolysin.


[img] Text

Download (401kB)

ID Number



View in Google Scholar

Actions (login required)

View Item View Item


Downloads per month over past year