Isolation and preliminary identification of active compounds from the stem of leea indica (memali) using chromatographic technique / Naila Nabihah Mohd Azam

Leea indica is a medicinal plant that has been widely used as traditional remedy in
curing many diseases. This study focused on isolation and preliminary
identification of active compounds from the three different stem extracts of L.
indica that have anti-oxidant and anti-microbial properties. The isolated active
compounds from dichloromethane (DCM) extract at Rf 0.17 and Rf 0.25 was
identified as 9-0ctadecane and Aromatic compound after subjected to FTIR,
NMR and GC-MS. In DPPH radical scavenging activity analysis, all of the three
extracts exhibit anti-oxidant properties where only methanol (MeOH) extract is
the most anti-oxidative. The IC50 of MeOH extract is 1.14 ug/ml, which exceed
the IC50 of Ascorbic acid (standard) which only 1.12 ug/ml. The stem extracts of
this plant exhibit both anti-oxidant and anti-microbial properties. MeOH stem
extract showed the strongest anti-oxidant activity due to presence of phenolic
compound in the extract which known as strong radical scavengers. The stem of
L. indica was proved containing active compounds that have anti-oxidant and
anti-microbial properties.