Electrospun anti-inflammatory patch loaded with citrus limon (L.) extracts incorporated with dimethyl sulfoxide (Dmso) for accelerating wound healing

The four consecutive phases of haemostasis, inflammation, proliferation, and remodelling comprise the normal natural wound healing process. The proper sequence and duration of these phases are essential for effective healing. On the contrary, endotoxins and bacterial infections can prolong the inflammatory phase by elevating pro-inflammatory cytokines, including TNF-α and interleukin-1 (IL-1). Should this extension continue, there is a chance the wound will become chronic and never heal. Numerous studies are being carried out to expedite the wound healing process for endotoxins and bacterial infections. However, factors like bacterial resistance to antibiotics and their narrow spectrum have made it more difficult to treat patients accurately. Therefore, it is advised to choose an approach that combines antibacterial and anti-inflammatory qualities for wound healing. This study aims to provide susceptibility to the bacteria and broaden the spectrum of antibiotics for various bacteria to address these issues. To address these challenges, this study proposes an electrospun anti-inflammatory patch loaded with Citrus limon (L.) extract incorporated with Dimethyl Sulfoxide (DMSO) for accelerating the wound healing process. Two different solvents (i.e., 70% ethanol and 10% DMSO) will be loaded onto the extracted citrus limon to generate the electrospun anti-inflammatory patch. DMSO is added as a carrier when ethanol is used as the solvent to improve Citrus limon's ability to permeate into the bacterial cell membrane. This patch has strong anti inflammatory qualities and shows promise as a successful way to treat bacterial infections. This patch also has antioxidants, which can help shield body cells from harm. The effective development of the patch on eliminating germs and speed up wound healing, it will solve many chronic diseases linked to bacterial infections and improve wound healing, enabling us to treat wounds as soon as possible at our convenience.