Optimization on micropropagation for typhonium flagelliforme (rodent tuber) in different strength of MS media, liquid culture system and analysis of total phenolic content

Tyhponium flagelliforme or rodent tuber is a medicinal herb which commercially used as anticancer supplement in Malaysia. This plant has limited sources due to their short life cycle, where the rhizome becomes dormant during dry season. Since T.flagelliforme biomass is needed for further research and commercialization, it is crucial to provide continuous production of this plant biomass. In vitro propagation through plant tissue culture (PTC) technique offers a tool for continuous and large scale production of T.flagelliforme. The present research aimed to optimize micropropagation of T.flagelliforme for their continuous supply and conservation. Changes in strength of MS media in solid and liquid media on growth, biomass and chlorophyll content of T.flagelliforme were evaluated. Then, the best treatment was used to investigate the effects of different liquid culture system on T.flagelliforme growth rate, biomass and chlorophyll content. The effects of different growth conditions on Total Phenolic Content (TPC) in T.flagelliforme leaves were also studied. The explants were cultured in different strength of MS media with and without addition of gelrite for 6 weeks with 15 replications. Then, T.flagelliforme shoot clumos were cultured in different liquid culture systems; Conventional Liquid Culture (CLC), Constant Immersion System (CIS) and Temporary Immersion Bioreactor System (TIBS) for 4 weeks with 6 replicates. All of the treatments were arranged in completely randomized design (CRD). Four leaves samples of T.flagelliforme were collected from in vitro Solid Culture Media (SCM).