Effect of lipase addition in oily water clean up

The marine environment is open to large sources of toxic organic waste in the form of accidental oil spills. Lipase as we know is class of enzymes that break down dietary fats into smaller molecules which is called fatty acids S.Thakur (2005). Thus In this experimental study, the main objective is to see the effects of lipase towards the oily water and to observe the time period during the effects of lipase and the degree of hydrolysis. The oil for this experiment that used was olive oil since olive oil easily to have high reaction compare with other oil that meet with required. The preparation of the lipase solution were done at different concentration which at 2.0, 2.5 and 3.0 mg/ml.After that, 0.05 M concentration of olive oil was prepared and mix with isooctane as solvent at 50 ml.The time period taken for the sample were at 2, 4 and 6 hours.Then using the titration method which to find the degree of hydrolysis by using 0.01 N of NaOH.The result show at the different concentration of lipase which were at 2, 2.5 and 3 mg/ml give a slightly difference value of the amount of NaOH used.This show the sample was more acidic. From the result at period of 2 hours from the concentration of 2.0 mg/ml, the amount of the NaOH used was 0.5 ml while for 2.5 and 3 mg/ml were 1.1ml and 1.3 ml respectively. The result for time period also prove to be the main criteria to maximize the activity of lipase where as at 2 mg/ml with the time period at 6 hours the value of hydrolysis higher which was 2.26% follow by at 3 and 2.5 mg/ml the value of hydrolysis were 2.11% and 1.78% respectively.From there, the increase of time period cause the degree of hydrolysis to increase. The overall results indicate that lipase could be a promising toward the method to overcome the oil contaminant in near future.