Batch anaerobic fermentation of pomegranate fruit: time-axis analysis of species diversity

Batch anaerobic spontaneous fermentation based on a medium of 10% grinded pomegranate fruit and 10% sucrose in distilled water was carried out over a period of 12 weeks to study the species diversity along the time axis. In this research, streaking on nutrient agar, potato dextrose agar and a few selective media as well as Gram staining were applied to obtain the results. Before the fermentation samples are streaked on the various media, they were diluted in a series. The selective media used were MacConkey agar, Urea agar and MRS (de Man, Rogosa and Sharpe) agar. Potato Dextrose Agar (PDA) was used for the growth of fungus whereas nutrient agar was used for the total growth. Gram staining was carried out for the growth on the selective media to determine if the bacteria was Gram positive or Gram negative.