In Vitro study of Hydroxyapatite (HAp) specimen in the Simulated Body Fluid (1.5SBF) / Ainina Kamalrulzaman

This study had been conducted to determine In-Vitro properties of hydroxyapatite (HAp) specimen synthesize from the clamshells via precipitation method. HAp specimen were successfully prepared and then immersed in the simulated body fluid (SBF) which has a similar composition to the human blood plasma. 1.5SBF concentration has been chosen as fluid for In-Vitro studies. This research also highlights the purpose of In-Vitro studies of HAp specimen in order to verify the formation of apatite layer on the surface of HAp specimen after its immersion in the simulated body fluid in certain period of time up to 25 days. After immersion, the HAp specimen was then characterized by using Scanning Electro Microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDX) and Fourier Transform Infrared Spectroscopy (FTIR). The Fourier Transform Infrared Spectroscopy (FTIR) showed the intensity of functional groups in the HAp such as phosphate, carbonate and OH groups. While, by using Scanning Electro Microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDX), the morphology of apatite layer formed in the surface of Hap pellet was analysed and studied. The growth of apatite layers formed in the surface of HAp specimen after immersed in the 1.5SBF was showed as result of study.