Relative real-time polymerase chain reaction analysis of gene regulated by gelam honey in A549 lung cancer cell line / Shareena Khadmudin

In this study, we are looking at the differentially express gene of lung cancer cell line, which is the Human Lung Adenocarcinoma Epithelial Cell Line 549 (A549) which was treated with Gelam honey. Gelam honey is one the most valuable treasures in Malaysia and studies conducted so far has shown that honey contained compounds able to act as anticancer agents. The method used to analyze the relative gene expression was Real-time Polymerase Chain Reaction. Based on the results, Gelam honey caused genes to be differentially expressed.