Development of a PCR method for the detection of genetic polymorphism of BRCA1 / Nur Farhah Busyra Ismail

Breast cancer is one of the most common malignancies in women. It continues to be a major burden and cause of death among women worldwide. Several epidemiologic studies have identified risk factors for breast cancer, including a family history of the disease. There are several genes that have been reported associated with breast cancer and one of them is BRCAJ gene. BRCAJ gene is a key role that acts as suppressor gene which is inherited as an autosomal dominant. This study aims to develop a diagnostic test for genetic determination of breast cancer risk by detection of BRCAJ which may be associated with breast cancer. Thus, in this study, a set of primers was designed and polymerase chain reaction based detection method was developed to detect polymorphism of BRCA 1. It is important to study on the association of breast cancer with BRCAJ gene to help further development in clinical studies and improvement of quality of life in world population especially Asian country.