Isolation and purification of damnacanthal and nordamnacanthal from dichloromethane fractions of roots of morinda citrifolia and its neurotoxicity and neuroprotection effects / Izdihar Mohd Noor

Morinda citrifolia is said to have countless biological activity. Damnacanthal and nordamnacanthal listed under anthraquinones group which were identified to be the major compounds in roots of Morinda citrifolia. Isolation and purification of both compounds were the interest in this research. 50:50 dichloromethane:hexane solvent was used as the mobile phase in column chromatography packed with acid-washed 1 I Q silica to isolate and purify both damnacanthal and nordamnacanthal. H and C NMR were carried out to elucidate on the structure of both compounds collected. Besides that, the investigation of other biological activity of both damnacanthal and nordamnacanthal were conducted. Solvent toxicity, IC50 of hydrogen peroxide toxicity, neurotoxicity and neuroprotection assays were done on SH-SY5Y cell by conducting cell viability test of MTT. The result of neurotoxicity and neuroprotective effect of both compounds were determined by the percentage of cell viability of the SH-SY5Y cell.