Investigation of in vitro metabolism of carbamazepine using CYP2C9 enzymes through 1H-NMR spectroscopic method / Raudhatun Sa' Adiah Ithnin

The main objective of this in vitro study were plan to identify major Carbamazepine metabolites formed in association with CYP2C9 enzymes respectively using variable such as different concentration of Carbamazepine, pH of medium and concentration of CYP2C9 enzymes. Other goals were to identify the structural changes during metabolism of Carbamazepine compound. There are several techniques involved in order to achieve the goals of this project. The first process involves enzymatic reactions of Carbamazepine. The reaction samples were subjected to extract and evaporate to get pure extract. Obtained extract were further subjected to Thin Layer Chromatographic (TLC) techniques in order to identify transformed metabolites. The suspected metabolites detected on TLC plates were sent for characterization as long as identification using 1H-NMR spectrometer. Two compounds were detected namely, compound (2) and (3) respective to pH 5.8 and 7.4. Concisely, even though the compounds are not main metabolites in metabolism of Carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of Carbamazepine.