Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak

Ghazali, Ahmad Rohi and Nuddin, Julenah Ag and Sabireen, Siti Nurfarhana and Wan Razali, Wan Marahaini (2013) Ex vivo antiplasmodial activity of Quassia Borneensis Noot. (SIMAROUBACEAE) extracts against mice erythrocytes infected by Plasmodium Berghei NK 65 / Ahmad Rohi Ghazali, Julenah Ag Nuddin, Siti Nurfarhana Sabireen, Wan Marahaini Wan Razali and Shafariatul Akmar Ishak. International Journal of Undergraduates Studies, 2 (2). pp. 24-31. ISSN 2289-4242

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Abstract

This study is attempts to determine antimalarial activity of Q. borneensis extracts against the intraerythrocytic phase of P. berghei NK65. Parts of Q. borneensis was extracted by cold maceration and soxhlet in 10% aqueous methanol before partitioned with n-Hexane, and chloroform. The extratcs were subjected to colorimetric Plasmodium Lactate Dehydrogenase Assay (pLDH). The extract with most potential IC50 was later evaluated in synchronization test. It was found that hexane soxhlet extract of root, (Qb4), and chloroform soxhlet extract of bark, (Qb7) showed potential, at the values of IC50 0.039μg/ml and 0.001μg/ml, respectively. In the synchronization test, Qb7 extract was found to be active against all three stages of parasites in the intraerythrocytic phase, with the IC50 value for the early, late trophozoite and schizont at 1.964 μg/ml, 0.0009μg/ml and 0.007 μg/ml respectively. This study coulclude that Q. borneensis might possess biologically active quassinoids which could contribute to their antimalarial activities.

Item Type: Article
Creators:
CreatorsEmail
Ghazali, Ahmad RohiUNSPECIFIED
Nuddin, Julenah AgUNSPECIFIED
Sabireen, Siti NurfarhanaUNSPECIFIED
Wan Razali, Wan MarahainiUNSPECIFIED
Subjects: Q Science > QD Chemistry > Organic chemistry
R Medicine > RC Internal Medicine > Chronic diseases > Malaria
Divisions: Faculty of Business and Management
Journal or Publication Title: International Journal of Undergraduates Studies
ISSN: 2289-4242
Volume: 2
Number: 2
Page Range: pp. 24-31
Item ID: 8749
Uncontrolled Keywords: Simaroubaceae, Quassia, pLDH assay, antimalarial activity
Last Modified: 03 Aug 2017 04:03
Depositing User: Staf Pendigitalan 1
URI: http://ir.uitm.edu.my/id/eprint/8749

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