The effect of Ficus Deltoidea’s fruit extraction in different concentration on mouse oocyte / Faezah Ibrahim

Superovulation of female mice with exogenous gonadotrophins is routinely used for
increasing the number of eggs ovulated by each female in reproductive and
developmental studies. Superovulation is affected by many factors such as hormone
concentrations, types of hormone, species of animals, age, time of administrations and
many others. Previous reports gave the emphasis on hormone and it dosage. This study
was designed to investigate whether the different in concentration of Ficus deltoidea’s
fruit extraction can replaces the function of Pragnant Mare’s Serum Gonadotropin to
mimic the endogenous effects of follicle stimulating hormones (FSH) in order
superovulation process can occur. Ficus deltoidea is popular among medical healer and
local people to treat some illness and disease. Based on traditional knowledge, Ficus
deltoidea leaves posses characteristics of human reproductive organ and in particular for
the female fertility treatment. The different concentrations of Ficus deltoidea’s fruit
extracts were given to a different group of mice in a week time and the procedure
proceeds with Human Chorionic Gonadotropin (hCG) injection. Fourteen hours later,
oocyte retrieval was conducted in the laboratory. After the oocyte retrieval, the oocyte
quality and quantity from all concentrations were observed. The oocyte quality and
quantity observed includes the total number of oocyte retrieved and the oocyte
morphology base on the standard basis. The finding showed that there were slight
decreased in oocyte quantity however all oocyte showed normal morphology. Other
factors that can influence the result were the strain, age of mice and the environmental
factor