pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira

Valente Pereira, Rosa Elizabeth (2008) pH-sensitive nanoparticles as carriers for oral delivery of a model peptidomimetic drug / Rosa Elizabeth Valente Pereira. PhD thesis, Universiti Teknologi MARA (UiTM).

Abstract

Peptide-related drugs are usually unstable in the harsh gastrointestinal environment with poor absorption throughout the gut. The potential of anionic pH-sensitive nanoparticles to act as novel drug carriers for oral administration of such compounds was investigated using a peptidomimetic drug, cefotaxime sodium. Drug loaded nanoparticles were prepared using a pH-controlled nanoprecipitation method. Three types of nanoparticles were prepared using different grades of Eudragit polymer, namely LI00, LI00-55 and SI00. The drug-loaded nanoparticles have a size of about 100 nm, low polydispersity, encapsulation efficiencies of more than 50%, drug entrapment and drug content between 4-5%. Bioavailability studies were initially conducted in rats according to a crossover design. However, a carry-over effect was observed with visible reduction of cefotaxime absorption following the first exposure of the rats to the drug-loaded nanoparticles. This phenomenon was confirmed with repeated administration of the same drug-loaded nanoparticles to the same rats (after one week wash-out period). Thus, in subsequent studies, a parallel group design was used instead. The extent of cefotaxime bioavailability was found to increase in the following order with the different polymeric nanoparticles: S100>L100-55>L100. Bioavailability of the nanoparticles from isolated segments of rat intestines was further investigated to determine if they were preferentially absorbed from specific regions. Absorption was found to occur in the entire small intestine. However, the duodenum, jejunum and jejunum-ileum areas were observed to be the optimal sites for absorption of the L100, L100-55, and S100 nanoparticles respectively. Finally, the drug-loaded nanoparticles were also observed to enhance the lymphatic transport of the contained drug. The ratio of the cefotaxime concentration in the lymph over that of the plasma obtained with administration of the nanoparticles was consistently higher than that obtained with administration of an aqueous solution of the drug, suggesting that nanoparticles could promote the lymphatic transport of the drug.

Metadata

Item Type: Thesis (PhD)
Creators:
Creators
Email / ID Num.
Valente Pereira, Rosa Elizabeth
2004634701
Contributors:
Contribution
Name
Email / ID Num.
Thesis advisor
Abdul Majeed, Abu Bakar
UNSPECIFIED
Subjects: R Medicine > RM Therapeutics. Pharmacology > Drugs and their actions
R Medicine > RS Pharmacy and materia medica > Materia medica > Pharmaceutical dosage forms
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Pharmacy
Programme: Doctor of Philosophy
Keywords: Peptide-related drugs, gastrointestinal environment, peptidomimetic drug, cefotaxime sodium
Date: 2008
URI: https://ir.uitm.edu.my/id/eprint/5480
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