Isolation and characterization of bacteria associated with smoked catfish (order siluriformes: family chlariidae) in Kuala Pilah, Negeri Sembilan / Nurhidayah Yusof

Yusof, Nurhidayah (2016) Isolation and characterization of bacteria associated with smoked catfish (order siluriformes: family chlariidae) in Kuala Pilah, Negeri Sembilan / Nurhidayah Yusof. [Student Project] (Unpublished)

Abstract

Bacteria are a part of the microflora of the environment, which may cause harm to human. Bacteria are transmitted to human in a various ways, especially through food. One example of food that can transmit bacteria is smoked fish. Commonly, this type of food is found in an open shelf either in a proper outlet or a roadside stall. A study was conducted aiming at the isolation and characterization of bacteria associated on smoked catfish. Family Chlariidae fish was used as sample in the study. The smoked fish samples were collected at three different markets around Kuala Pilah, Negeri Sembilan. The bacteria were isolated by swabbing the catfish skin, and the samples were characterized by gram staining and biochemical tests. Differentiation and characterization of the isolates were based on their growth characteristics on various media, such as Mc Conkey Agar (MCA), Nutrient Agar (NA), Triple Sugar Iron Agar (TSI), Salmonella-Shigella Agar (SSA), Simmons Citrate Agar (SCA), Sulfide-Motility-Indole Medium (SIM) and Carbohydrate fermentation test. The mean bacteria load for smoked fish was 6.57 x 10-6 CFU/ml. The following bacteria isolated were Citrobacter sp., Escherichia sp., Enterobacter sp. and Shigella sp.

Metadata

Item Type: Student Project
Creators:
CreatorsID Num. / Email
Yusof, NurhidayahUNSPECIFIED
Contributors:
ContributionNameID Num. / Email
Thesis advisorYaacob, IlyanieUNSPECIFIED
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QR Microbiology > Bacteria
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Biology
Item ID: 34431
Uncontrolled Keywords: Isolation and characterization of bacteria, smoked catfish (order siluriformes: family chlariidae)
URI: http://ir.uitm.edu.my/id/eprint/34431

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