Molecular phylogeny of unidentified rats using partial Cytochrome B Fragment / Nur Syahirah Zakaria

Zakaria, Nur Syahirah (2018) Molecular phylogeny of unidentified rats using partial Cytochrome B Fragment / Nur Syahirah Zakaria. [Student Project] (Unpublished)

Abstract

Rattus spp. are widely distributed all over the world and most species cause damaged to habitat and the spreading of the disease. This species have evolved to adapt with their surrounding. This study aims to determine the species of unidentified rats based on phylogeny-based identifications using partial cytochrome b (cytb) fragment and to compare with the morphological identification of unidentified rats with phylogeny-based identifications. The physical characteristics of five unidentified rats caught were observed and genomic DNA from all five rats were extracted and amplified. Only three samples were successfully amplified and sequenced for a total of 342 bp. Phylogenetic relationships among these three sequences with additional of 71 Rattus spp. sequences were inferred from Neighbour-Joining, Bayesian inference and Maximum Likelihood analyses. Based on the phylogenetic analyses, the three unidentified rats were possibly related to R. Tiomanicus, R. Kandianus and/or R. rattus. It was difficult to distinguish the species of these rats based on their physical characteristics due to their similar appearance, hence a molecular data gave a clue of their possible species identification.

Metadata

Item Type: Student Project
Creators:
CreatorsID Num. / Email
Zakaria, Nur SyahirahUNSPECIFIED
Contributors:
ContributionNameID Num. / Email
Thesis advisorZakaria, Sarah ShazwaniUNSPECIFIED
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QD Chemistry > Extraction (Chemistry)
Q Science > QP Physiology > DNA. Deoxyribonucleic acids
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Biology
Item ID: 34337
Uncontrolled Keywords: Molecular phylogeny, unidentified rats, Cytochrome B Fragment
URI: http://ir.uitm.edu.my/id/eprint/34337

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