Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan

Mazlan, Siti Mas Nur Ain (2017) Isolation and identification of lactobacillus sp. from fresh fruits / Siti Mas Nur Ain Mazlan. [Student Project] (Unpublished)

Abstract

Lactic Acid Bacteria has been widely studied in dairy products. However, the presence of Lactic Acid Bacteria (LAB) in fruits has rarely been focused. Fruits contain organic acids and rich with nutrients such as minerals, carbohydrates, nitrogen compounds and a low pH environment that are suitable for the growth of Lactobacillus sp. The objectives of this study were to isolate and identify Lactobacillus sp. from fresh fruits by using molecular techniques which is Polymerase Chain Reaction (PCR) method. The white colony from MRS agar was isolated from banana and red dragon fruits. The phenotypic method was used to confirm the shapes and color of Lactic Acid Bacteria (LAB) which is Gram positive bacteria with rod or coccus shaped. The PCR results showed the identification of Lactobacillus sp. are at 216 bp by using the specific primer which is Lacto-16S-R and Lacto-16S-F. The results also indicate that Polymerase Chain Reaction (PCR) is more efficient to detect the strains of bacteria and give highly specific results.

Metadata

Item Type: Student Project
Creators:
CreatorsID Num. / Email
Mazlan, Siti Mas Nur AinUNSPECIFIED
Subjects: Q Science > QD Chemistry > Extraction (Chemistry)
Q Science > QR Microbiology > Bacteria
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Biology
Item ID: 32574
Uncontrolled Keywords: Isolation and Identification, of Lactobacillus sp. From Fresh Fruits, UiTM Negeri Sembilan
URI: http://ir.uitm.edu.my/id/eprint/32574

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