Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]

Mohd Amin, Indah and Mohamad Zain, Nurhayati and Ahmad, Vivi Noryati and Mat Daud, Dzulfakhrul Najwan and Abdullah Sani, Nabilah (2019) Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]. In: Proceedings of 9th Dental Student’s Scientific Symposium,Malaysia (Malaysia, 2019), 9th – 10th April 2019, Faculty Dentistry, Universiti Teknologi MARA.

Abstract

The aims of this study are to investigate antibacterial and antibiofilm activity of Rosa damascena (R.damascena) flower extract against Staphylococcus aureus (S. aureus). Method: The antibacterial activity was assessed with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The potential of R.damascena flower extract as an antibiofilm agent was evaluated using biofilm inhibition assay and biofilm eradication assay. Chlorhexidine (CHX) 0.12% was used as a positive control. The experiment was performed in triplicate and repeated four times independently. All the data obtained were analysed using SPSS version 23. A Kruskal-Wallis test followed by post-hoc Mann-Whitney U test and level of significance was set at P < 0.05. Results: From this study, R.damascena extract shows antibacterial activity with MIC 50 mg/ml and MBC 100 mg/ml. Treatment with 0.5 MIC of extract inhibited 43.2% of biofilm formation. The biofilm eradication assay shows that 60.9% (p<0.001) of biofilm was eradicated by the extract upon 5 minutes treatment and 50.65% by 0.12% CHX. Conclusion: The result of this in-vitro study suggests a possible utilisation of R.damascena flower extract in treating S. aureus biofilm-associated infection in the oral environment.

Metadata

Item Type: Conference or Workshop Item (Paper)
Creators:
CreatorsID Num. / Email
Mohd Amin, IndahUNSPECIFIED
Mohamad Zain, NurhayatiUNSPECIFIED
Ahmad, Vivi NoryatiUNSPECIFIED
Mat Daud, Dzulfakhrul NajwanUNSPECIFIED
Abdullah Sani, NabilahUNSPECIFIED
Subjects: R Medicine > RM Therapeutics. Pharmacology > Drugs and their actions > Individual drugs and other agents
R Medicine > RS Pharmacy and materia medica > Materia medica
Divisions: Universiti Teknologi MARA, Selangor > Sungai Buloh Campus
Event Title: Proceedings of 9th Dental Student’s Scientific Symposium,Malaysia (Malaysia, 2019)
Event Dates: 9th – 10th April 2019
Page Range: pp. 1-4
Official URL: https://dentistry.uitm.edu.my/v2/images/images/DSS...
Item ID: 30163
Uncontrolled Keywords: Denture-induced stomatitis (DIS), Denture cleansers, therapeutic agents
URI: http://ir.uitm.edu.my/id/eprint/30163

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