Phytochemical analysis and bioactivity studies of ziziphus mauritiana (twigs and leaves) / Saidatul Aqilah Mohamad Yusof and Rohaiza Saat

Mohamad Yusof, Saidatul Aqilah and Saat, Rohaiza (2017) Phytochemical analysis and bioactivity studies of ziziphus mauritiana (twigs and leaves) / Saidatul Aqilah Mohamad Yusof and Rohaiza Saat. Journal of Academia, 5 (1). pp. 17-26. ISSN 2289-6368

Abstract

Malaysia lack and inadequate research about Ziziphus mauritiana. Two part of Z. mauritiana plant obtained which are leaves and twigs for this study. Z. mauritiana extraction processes conducted using three difference polarity of solvents which are hexane, chloroform and methanol by cool extraction method. The highest yield percentage is chloroform leaves extract which is 3.71%. The phytochemical analysis study revealed that many secondary metabolites presence inside Z. mauritiana such as alkaloid, flavonoid, glycoside, phenol, saponin, steroid, sterol, tannin and terpenoid but for leave part only absence of saponin detected. In thin layer chromatography (TLC) method, the mixture of solvent system used are hexane and chloroform with ratio 3:7 or 1:9 to obtain good separation of compound under short (254 nm) and long (366 nm) wavelength UV lamp. Antibacterial activity examined using disc diffusion method toward Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Salmonella sp. conducted. The highest maximum zone inhibition diameter recorded is hexane leaves crude extract on S. aureus bacteria which is 15 mm. In addition, antioxidant study discovered the highest percentage found on crude extract of methanol.

Metadata

Item Type: Article
Creators:
CreatorsID Num. / Email
Mohamad Yusof, Saidatul AqilahUNSPECIFIED
Saat, Rohaizarohaizas@ns.uitm.edu.my
Subjects: Q Science > QK Botany > Plant physiology > Botanical chemistry. Phytochemicals
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Journal or Publication Title: Journal of Academia
Journal: UiTM Journal > Journal of Academia UiTM Negeri Sembilan
ISSN: 2289-6368
Volume: 5
Number: 1
Page Range: pp. 17-26
Item ID: 29359
Uncontrolled Keywords: Ziziphus mauritiana, Phytochemical analysis, Thin layer chromatography, Antibacterial, Antioxidant
URI: http://ir.uitm.edu.my/id/eprint/29359

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