Preliminary screening on the effect of persicaria minor (kesum) against haemonchus contortus / Nik Noor Haszwani Yahya

Yahya, Nik Noor Haszwani (2017) Preliminary screening on the effect of persicaria minor (kesum) against haemonchus contortus / Nik Noor Haszwani Yahya. [Student Project] (Unpublished)

Abstract

Haemonchus contortus is a type of nematode that is commonly infecting the sheep which causes hemorrhagic anemia or even death towards sheep. This gastrointestinal parasite could also develop resistance towards synthetic antihelminthics, leads to the study in finding the alternative antihelminthics. So, the antihelminthic activity of Kesum against H. contortus was investigated. Kesum plant was dried and soaked in ethanol to obtain the ethanolic Kesum extract. The phytochemical assays (tannins, steroids, flavonoids, alkaloids and saponins) were done towards the extract. Preliminary screening by using egg hatch assays (EHA) and larval development assays (LDA) were done in triplicate form with different extracts concentrations (50, 25, 12.5, 6.25, 3.125 mg/mL) to study the efficacy inhibition percentage of the egg and larval development of H. contortus by Kesum ethanolic extract. 0.125 mg/mL of Albendazole used as positive control and 1 % DMSO and 95% ethanol as negative control. Kesum ethanolic extracts shows presence of all the phytochemical contents. During the experiment low number of eggs and larvae obtained, which does not fall in the range of 100 eggs/0.2 mL egg suspensions. In spite of that, Kesum extracts were clearly identified to exhibit larvicidal properties against H. contortus as observed in LDA. Thus, it can be stated that Kesum has the possibility to inhibiting the development of H. contortus. Further study towards the antihelminthic activity of Kesum against H. contortus should be done and followed by the identification of phytochemicals in Kesum that affects the development of H. contortus.

Metadata

Item Type: Student Project
Creators:
CreatorsID Num. / Email
Yahya, Nik Noor HaszwaniUNSPECIFIED
Contributors:
ContributionNameID Num. / Email
Thesis advisorIberahim, RashidahUNSPECIFIED
Subjects: Q Science > QD Chemistry > Extraction (Chemistry)
Q Science > QR Microbiology
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Biology
Item ID: 28479
Uncontrolled Keywords: persicaria minor, kesum, haemonchus contortus, UiTM Negeri Sembilan
URI: http://ir.uitm.edu.my/id/eprint/28479

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