Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar

Wan Hanafi, Wan Nurhayati and Abu Bakar, Nur Syahirah (2019) Application of Whatman FTA card method in oil palm DNA extraction and PCR analysis / Wan Nurhayati Wan Hanafi and Nur Syahirah Abu Bakar. Scientific Research Journal, 16 (2). pp. 17-26. ISSN 1675-7009

Abstract

Polymerase Chain Reaction (PCR) analysis in the study of oil palm DNA generally carried out by using DNA template obtained from grinding of leaf samples in liquid nitrogen followed by hexadecyltrimethylammonium bromide (CTAB) protocol. The present study explores the FTA card as a method to retrieve PCR-amplifiable oil palm DNA. Oil palm leaves were cut and crushed before deposited onto the FTA card. An attempt was made by amplifying the EgSHP gene using a punch of FTA card as a DNA template. The successful outcome of PCR was measured by the presence of PCR amplicons on 1% agarose gel electrophoresis, indicate the genotype of oil palm fruit form. This present study demonstrates that the FTA card provides a versatile alternative to the study of oil palm genetics.

Metadata

Item Type: Article
Creators:
CreatorsID Num. / Email
Wan Hanafi, Wan Nurhayatiwannurhayati@uitm.edu.my
Abu Bakar, Nur SyahirahUNSPECIFIED
Subjects: Q Science > QP Physiology > Animal biochemistry
Q Science > QP Physiology > DNA. Deoxyribonucleic acids
Divisions: Universiti Teknologi MARA, Shah Alam > Research Management Centre (RMC)
Journal or Publication Title: Scientific Research Journal
Journal: UiTM Journal > Scientific Research Journal
ISSN: 1675-7009
Volume: 16
Number: 2
Page Range: pp. 17-26
Item ID: 28261
Uncontrolled Keywords: Oil palm, FTA, PCR
URI: http://ir.uitm.edu.my/id/eprint/28261

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