Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun

Harun, Nur Sakinah (2015) Potential pitfall in cytological diagnosis: Cytomorphology of tropical fruits and local grains in Malaysia / Nur Sakinah Harun. Degree thesis, Universiti Teknologi MARA.

Abstract

Introduction: Pitfall is known as misdiagnosing or misinterpretation that can lead to whether false positive or false negative result, and may affect treatment, thus lead to morbidity. Error in misdiagnosis can contribute to false treatment and may lead to death. Possible contaminants in cytomorphological study, including food particles such as fruits and grains may cause pitfall by mimicking certain human cells or fungal element that present in human, and staining background. Objectives: Purposes of this study are to identify cytomorphology of tropical fruits and local grains, which may cause pitfalls in cytological diagnosis, and to compare and recognize the mimicking morphological characteristics of the selected fruits and grains cells to cells in cytology. Method: Random tropical fruits and local grains were selected as samples. The samples were homogenized using mortar and pestle, and smeared onto glass slides. For each sample, two slides were prepared, one slide was fixed into 95% alcohol, and another one was air-dried. The slides were then stained using Papanicolaou and May Grunwald's Giemsa respectively. Stained slides were observed for cells morphologies and background. Selected cells morphologies were then captured using Leica DM750 Microscope with ICC 50HD camera. Results: Dragon fruit cells showed resembling criteria of benign lipoma and squamous epithelial cell, where mango fruit cells resemble cryptococcosis and fat droplet in adipocyte tissue. Ambarella fruit cells showed 'honeycomb' characteristics of endocervical cell. Starfruit cells and watermelon fruit cells showed resemblances to squamous epithelial cell and hyperkeratosis respectively, where maize cells resemble haematoxylin scum. Conclusion: The morphological characteristics of tropical fruits and local grains can resemble morphology of human cells and staining background. These findings can be used as references in cytological diagnosis, thus avoiding the potential pitfalls.

Metadata

Item Type: Thesis (Degree)
Creators:
CreatorsID Num. / Email
Harun, Nur SakinahUNSPECIFIED
Subjects: Q Science > QH Natural history - Biology > Cytology
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Health Sciences
Programme: Bachelor of Medical Laboratory Technology
Item ID: 28082
Uncontrolled Keywords: Pitfall, cytological diagnosis, tropical fruit, local grain, cytomorphology
URI: http://ir.uitm.edu.my/id/eprint/28082

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