Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin

Nordin, Nordiana (2015) Detection of DEL phenotype in RhD negative blood by freeze-thaw elution technique and sequence-specific prime polymerase chain reaction / Nordiana Nordin. Degree thesis, Universiti Teknologi MARA.

Abstract

The weakest weak D called DEL phenotype was able to be detected via serological adsorption elution and molecular genotyping. In this study, the freeze-thaw elution and ssp-peR were used to detect the DEL phenotype. A total of 43 RhD negative samples collected from the National Blood Centre were serologically tested for Rh phenotyping and screened for DEL phenotype by both serological freeze-thaw elution and SSP-PCR. Based on the Rh phenotyping, the 43 RhD negative samples consisted of 34 ccee phenotype, 6 Ccee phenotype, 2 ccEe phenotype and 1 CCee phenotype. No DEL phenotype was detected by serological freeze-thaw elution but 2.33% (1/43) from the samples were detected for DEL phenotype that carried the RHD1227A allele by SSP-PCR. As a conclusion, the aim of this study was accomplished by the ability to detect DEL phenotype via molecular genotyping technique in distinguishing the DEL phenotype from truly D negative but not for freeze thaw elution technique.

Metadata

Item Type: Thesis (Degree)
Creators:
CreatorsID Num. / Email
Nordin, NordianaUNSPECIFIED
Subjects: R Medicine > RB Pathology > Clinical pathology. Laboratory technique
R Medicine > RB Pathology > Clinical pathology. Laboratory technique > Examination of the blood
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Health Sciences
Item ID: 27365
Uncontrolled Keywords: RhD negative, DEL phenotype, Malaysian population
URI: http://ir.uitm.edu.my/id/eprint/27365

Download

[img] Text
TD_NORDIANA NORDIN HS 15_5.pdf

Download (1MB)

Fulltext

Fulltext is available at:
UNSPECIFIED

ID Number

27365

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year