Synergy effect of salvadora persica and olea europaea twig extract based on phytochemical study, thin layer chromatography, and GC-MS profiling / Hayati Mawaddah Dzulkefli

This study is designed to explore the phytochemicals, thin layer chromatography
and GC-MS profiling of mixture between S. persica and 0. europaea twig
sample. The method used to in this study started by obtaining crude extracts by
using three solvents of different polarity; hexane (non-polar solvent), ethyl acetate
(medium polar solvent) and ethanol (polar solvent). The highest percentage yields
were recorded in ethanol (EtOH) extract with 14.5%, followed by ethyl acetate
(EtOAc) with 4.5% and hexane (HEX) with 0.79%. The detection and
identification of the phytochemical were performed through phytochemical
screening. The phytochemical analysis of hexane extract of mixture S. persica and
0. europaea twig showed the presence of saponins and terpenoid while for
ethanol and ethyl acetate extract only showed the presence of terpenoid
compound. The best developing solvents of thin layer chromatography (TLC)
analysis of hexane extract was 3:7 (EtOAc: HEX), ethyl acetate extract was 3:7
(EtOAc: CHCI3) and ethanol extract was 3:7 (EtOR: CRCI3). From TLC study,
the presence of saponin, terpenoid, alkaloid and phenolic were observed. GC-MS
detected some of compound from two different solvent used which is hexane
extract and petroleum ether extract. Finally, it has been suggested that the
synergistic activity S.persica and o.europaea study should be continue with
antibacterial activity research that will widely use in market.