Antioxidant activity on Oryza sativa (Paddy) and thier phytochemical screening and TLC profiling / Nor Hidayah Abu Bakar

Abu Bakar, Nor Hidayah (2019) Antioxidant activity on Oryza sativa (Paddy) and thier phytochemical screening and TLC profiling / Nor Hidayah Abu Bakar. [Student Project] (Unpublished)

Abstract

In the present study, the phytochemical screening, TLC profiling and antioxidant activity were investigate in the two common Oryza sativa variety in Malaysia which MR220 CL2 and MR219. Farmer in Malaysia usually cultivate paddy from variety MR220 CL2 and MR219 because it resistant to the herbicide and have the higher yield. The both variety are planted more than 20 season in more than 90% of the planted area in Malaysia. This study aim to determine the presence of phytochemical screening, best system solvent toward TLC profiling and antioxidant activity. Six sample such as leaves, hull, cuticle, root, stem and panicle were extract with methanol from both variety and use for the further analysis of the presence alkaloid, saponin, flavonoid, tannin and terpenoid for phytochemical screening. TLC study was carried out by using various solvent system of varying polarity of hexane, chloroform, ethyl acetate, and methanol extract. Therefore, antioxidant activity was performed by DPPH (1, 1diphenyl-2-picryl hydrazyl) radical scavenging method for different extracts of Oryza sativa using different concentration solvent (50,100,150,200,220 and 500 ppm) and Vitamin C and BHA are used as positive antioxidant control. The result showed that leaves and panicle from the both variety have most of the phytochemical screening and thin layer chromatographic that constituted different polarity compounds with different Rf values. The DPPH free radicals were scavenged by all plant extracts in a concentration that depend on the IC50 value. It appear that all paddy extract have not active in antioxidant activity.

Metadata

Item Type: Student Project
Creators:
Creators
Email / ID Num.
Abu Bakar, Nor Hidayah
UNSPECIFIED
Subjects: S Agriculture > S Agriculture (General) > Agricultural ecology (General)
S Agriculture > S Agriculture (General) > Agriculture and the environment
Divisions: Universiti Teknologi MARA, Melaka > Jasin Campus > Faculty of Plantation and Agrotechnology
Programme: Bachelor of Science (Hons) Plantation Technology and Management (AT220)
Keywords: Oryza sativa; Phytochemical screening; TLC profiling; Antioxidant; DPPH
Date: 2019
URI: https://ir.uitm.edu.my/id/eprint/24390
Edit Item
Edit Item

Download

[thumbnail of 24390.pdf] Text
24390.pdf

Download (14kB)

Digital Copy

Digital (fulltext) is available at:

Physical Copy

Physical status and holdings:
Item Status:

ID Number

24390

Indexing

Statistic

Statistic details