Investigation of antioxidative constituents from the stem of entada spiralis / Norshazleen Mohd Azenan

This study is about the determination of antioxidant activity in the stem of E.
spiralis. Phytochemical screening test was performed by using the methanol
extraction. Then, the methanol extract was proceed to determine the functional
group using FTIR. Thin Layer Chromatographic (TLC) Analysis was developed
to identify the compound that presence in the stem of E. spiralis. Each TLC was
sprayed by using spraying reagent to identify compound that contains in each
extract. After the extraction of methanol, dichloromethane, and petroleum ether
was done, the best solvent system was used to developed TLC analysis. The best
solvent system for MeOH extraction is 27:3 (HEX:EA), DCM extraction is
29.4:0.6 (DCM:MeOH), and PE extraction is 24:6 (Chloroform :Acetone). Then,
serial dilution was conducted to investigate the antioxidative assay in the stem of
E. spiralis and also identify IC50. Methanol extract has the highest antioxidant
activity. Isolation of antioxidative compound from PE extract from different Rf
then proceed with GC-MS analysis. Oxiraneoctanoic acid, 3-octyl-, cis, 9,12 Octadecadienoic
acid, Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl
ester, Butylaldehyde, 4-benzyloxy-4-[2,2,-dimethyl-4-dioxolanyl], Butylaldehyde,
4-benzyloxy-4-[2,2,-dimethyl-4-dioxolanyl], Hexadecanoic acid, methyl ester,
IH-Indene, I-methylene, Benzene, 1,2,4,5-tetramethyl, and trans-Verbenyl
isovalerate have been isolated and identified by GC-MS.